Dr. Joana Ferreira
Interdisciplinary Institute for Neuroscience (IINS), University of Bordeaux, Bordeaux, France
Dr. Juan Varela
Department of Chemistry, University of Cambridge, Cambridge, UK
Dr. Melike Lakadamyali
ICFO-Institute of Photonic Sciences, Castelldefels (Barcelona), Spain
“Super-resolution imaging of autoimmune encephalitis”
Dr. Joana Ferreira
Interdisciplinary Institute for Neuroscience (IINS), CNRS 5297, University of Bordeaux, Bordeaux, France
“Single nanoparticle tracking of neurotransmitter receptors in cultured and intact brain tissue”
Prof. István Katona
Momentum Laboratory of Molecular Neurobiology, Department of Molecular and Developmental Neurobiology, Institute of Experimental Medicine (KOKI), Hungarian Academy of Sciences (MTA), Budapest, Hungary
"Cell-type-specific STORM super-resolution imaging of synaptic endocannabinoid signaling"
Dr. Juan Varela
University of Cambridge, Department of Chemistry, Cambridge, UK
“Single-molecule imaging of protein aggregates in cerebrospinal fluid of Parkinson’s disease patients”
In recent years a large amount of work has been done to improve our ability to image individual molecules with high spatial and temporal resolution. Single-molecule detection and tracking has become a powerful tool to study the dynamics of biological events, as recognized by the Chemistry Nobel Prize in 2014. The observation of individual molecules eliminates implicit averages of conventional microscopy, allowing the mapping of heterogeneity in biological specimens and the observation of dynamic fluctuations and diffusion processes at the molecular level. Single-molecule imaging techniques have significantly contributed to developments in the neuroscience field, as many of the key biological structures are organized at the nano-scale. Super-resolution microscopy has shed new light on the molecular organization of the synapse and the regulation of neuronal communication. These new advances are especially important if we are to decipher currently unanswered questions on neurological pathologies, such as neurodegenerative diseases, brain encephalitis or schizophrenia.
Although super-resolution tools are becoming popular in neuroscience, single-molecule imaging techniques have only recently been applied in complex environments such as brain slices or bio-fluids. The first aim of this symposium is to bring together recent developments in single-molecule imaging applied to physiologically relevant scenarios, ranging from brain slices to cerebrospinal fluid. It is critically important to be able to develop and adapt these techniques in such a way that they can be used to unravel molecular information with minimal bias from the sample preparation. The second aim is to present specific applications of single-molecule imaging to study molecular mechanisms in physiological or disease contexts. With this in mind, the session will focus on successful examples of single-molecule imaging techniques that have recently yielded new insights into human physiology and disease.
The speakers will exploit novel imaging methods (e.g. vividSTORM, PALM, PAINT and single-nanoparticle tracking) to study both normal physiology and disease (with particular attention to autoimmune encephalitis and Parkinson’s disease). Due to its transversal character, the session will certainly be valuable for neuroscientists that want to apply single-molecule strategies in their own research domains.